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Full dent. sci ; 7(25): 123-129, jan.2016. ilus, tab
Article in Portuguese | LILACS | ID: lil-790088

ABSTRACT

O objetivo desta pesquisa in vitro foi avaliar a limpeza proporcionada pelo desbridamento no terço apical através da microscopia óptica e de varredura. Para isso, utilizaram-se cinquenta molares inferiores humanos, os quais depois de acessados foram impermeabilizados, esterilizados, contaminados com cepas de Enterococus faecalis (ATCC 29212), durante trinta e cinco dias. Posteriormente foram submetidos ao preparo químico-mecânico com as limas do sistema rotatório Protaper e desbridados com as limas #10 (G1); limas #15 (G2); limas #10 + medicação intracanal - MIC (G3); limas #15 + MIC (G4) e o controle positivo - sem desbridamento e sem MIC - (G5). Em seguida, os espécimes foram avaliados pelos métodos da microscopia óptica (clínica operatória) e microscopia eletrônica de varredura. O teste Kruskal-Wallis mostrou a eficiência do desbridamento, e verificou-se que houve diferença estatisticamente significante (p=0,000) entre os grupos G1 e o G2 no tocante ao aumento da eficácia do desbridamento radicular apical. Quanto às metodologias empregadas, observou-se que ambas são indicadas para avaliar a limpeza proporcionada pelo desbridamento, sendo que as análises através da MCO apresentaram imagens heterogêneas, enquanto a MEV imagens homogêneas. Pode-se concluir que o desbridamento foi eficaz na limpeza do terço apical em molares humanos, porém não houve diferença quanto ao calibre das limas #10 e #15. Os métodos de avaliação da microscopia óptica (clínica operatória) e microscopia eletrônica de varredura foram eficientes em visualizar a limpeza no terço apical...


The aim of this in vitro study was to evaluate the level of cleanliness achieved by debridement of the apical third of root canals by light and scanning microscopy. A total of 50 human mandibular molars were accessed, impermeabilized, sterilized and, subsequently inoculated with a strain of Enterococcus faecalis (ATCC 29212) for 35 days. They were then subjected to chemical-mechanical preparation with the following rotatory Protaper system files: Kerr files #10 (G1) and #15 (G2); Kerr files #10 + intracanal medication - MIC (G3); Kerr files #15 + MIC (G4); and positive controls without debridement or MIC (G5). The apical thirds of the canals were then examined by light clinical microscopy and scanning electron microscopy (SEM). The Kruskal-Wallis test showed the efficiency of the debridement, as verified by statistically significant differences (p=0.000) between groups G1 and G2 with regard to the increase in the effectiveness of the debridement apical radicular. Both light microscopy and SEM were suitable for assessing the cleanliness obtained by debridement. However, while the images seen under the light microscopy were heterogeneous, scanning electron microscopy yielded images that were homogenous. Debridement was efficient for cleansing the apical thirds of canals in human molars, with similar levels of cleanliness achieved with either Kerr files #10 or Kerr files #15. Both clinical microscopy and scanning electron microscopy allowed for adequate visualization for the evaluation of cleanliness in the apical third of the root canal...


Subject(s)
Humans , Tooth Apex/anatomy & histology , Molar/anatomy & histology , Debridement/methods , Microscopy, Electron, Scanning/methods , Root Canal Therapy , Odontometry/methods , Statistics, Nonparametric , In Vitro Techniques/methods
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